To start, try a high level API approach via a GUI… Using a GUI Saving and preserving metadata is key in quantitative image analysis. Metadata is essential to correctly read image data for example, to have accurate measurements, the image needs to be calibrated according to the correct/associated pixel size. metadata: information on the image data including pixel size, bit depth, dimension and objective information, etc.image data: which is essentially ‘pixel values’.When acquiring images in microscopy, the image files that are stored contain two main things:
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